Human Tumor necrosis factor receptor superfamily member 6B(TNFRSF6B) ELISA kit

Human Tumor necrosis factor receptor superfamily member 6B(TNFRSF6B) ELISA kit

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Mfr.No.

CSB-EL023982HU

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor receptor superfamily, member 6b, decoy, hCG_2044836, DCR3, DJ583P15.1.1, M68, TR6, OTTHUMP00000031583, decoy receptor 3, tumor necrosis factor receptor superfamily, member 6b

Recovery

The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	104
	Range %	98-110
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.516	2.587	2.552	2.380
500	2.021	2.106	2.064	1.892
250	1.362	1.324	1.343	1.171
125	0.761	0.739	0.750	0.578
62.5	0.503	0.534	0.519	0.347
31.25	0.375	0.359	0.367	0.195
15.6	0.276	0.295	0.286	0.114
0	0.174	0.170	0.172

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O95407

Applications	ELISA
Research Area	TNFRSF6B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504731	CSB-EL023982HU	96T	$695	Add to Cart
BHE10504731	CSB-EL023982HU	5x96T	$3336	Add to Cart
BHE10504731	CSB-EL023982HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.