Human Tumor necrosis factor ligand superfamily member 18(TNFSF18) ELISA kit

Human Tumor necrosis factor ligand superfamily member 18(TNFSF18) ELISA kit

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Mfr.No.

CSB-EL023993HU

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Alternative Names

tumor necrosis factor (ligand) superfamily, member 18, AITRL, GITRL, MGC138237, TL6, hGITRL, AITR ligand, GITR ligand, glucocorticoid-induced TNFR-related protein ligand

Recovery

The recovery of mouse IFN-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	99	92-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-99
1:2	Average %	97
	Range %	87-105
1:4	Average %	95
	Range %	89-100
1:8	Average %	90
	Range %	82-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.889	2.785	2.837	2.670
500	2.356	2.324	2.340	2.173
250	1.571	1.614	1.593	1.426
125	0.885	0.862	0.874	0.707
62.5	0.569	0.542	0.556	0.389
31.2	0.315	0.330	0.323	0.156
15.6	0.254	0.247	0.251	0.084
0	0.164	0.169	0.167	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

UniProt ID	Q9UNG2

Applications	ELISA
Research Area	TNFSF18

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504719	CSB-EL023993HU	96T	$695	Add to Cart
BHE10504719	CSB-EL023993HU	5x96T	$3336	Add to Cart
BHE10504719	CSB-EL023993HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.