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Home / ELISA Kit

Human Telomerase reverse transcriptase(TERT) ELISA kit

SKU : BHE10504512

This Mouse ICAM1 ELISA Kit was designed for the quantitative measurement of Mouse ICAM1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EL023391HU

Reactivity

Human

Sample Type

Serum, Plasma, Cell Culture Supernates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		82-101
EDTA plasma (n=4)		94		86-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		91-105
1:2		Average %		85
		Range %		80-92
1:4		Average %		96
		Range %		85-105
1:8		Average %		93
		Range %		83-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5000	2.555	2.518	2.537	2.390
2500	2.047	2.105	2.076	1.929
1250	1.531	1.552	1.542	1.395
625	0.812	0.803	0.808	0.661
312	0.516	0.524	0.520	0.373
156	0.312	0.321	0.317	0.170
78	0.229	0.225	0.227	0.080
0	0.145	0.149	0.147

Species

Homo sapiens

Limit Of Detection

450 nm

UniProt ID	O14746
Antigen	Telomerase Reverse Transcriptase

Applications

ELISA

Research Area

TERT

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10504512	CSB-EL023391HU	96T	$695		Add to Cart
BHE10504512	CSB-EL023391HU	5x96T	$3336		Add to Cart
BHE10504512	CSB-EL023391HU	10x96T	$6602.50		Add to Cart

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