Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA kit

Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA kit

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Mfr.No.

CSB-E16845h

Sensitivity

15.6 pg/ml

Detection Range

62.5 pg/ml - 4000 pg/ml

Reactivity

Human

Assay Duration

1-5h

Alternative Names

superoxide dismutase 1, soluble, ALS, ALS1, IPOA, SOD, homodimer, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase, cystolic

Recovery

The recovery of pig KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	88
	Range %	84-92
1:4	Average %	90
	Range %	85-98
1:8	Average %	106
	Range %	101-114

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.815	1.731	1.773	1.632
100	1.128	1.173	1.151	1.010
50	0.789	0.827	0.808	0.667
25	0.503	0.526	0.515	0.374
12.5	0.312	0.309	0.311	0.170
6.25	0.251	0.271	0.261	0.120
3.12	0.188	0.178	0.183	0.042
0	0.144	0.138	0.141

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P00441

Applications	ELISA
Research Area	SOD1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504462	CSB-E16845h	96T	$790	Add to Cart
BHE10504462	CSB-E16845h	5x96T	$3792	Add to Cart
BHE10504462	CSB-E16845h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.