Human squamous cell carcinoma antigen 1 (SCCAg1) ELISA kit

Mfr.No.

CSB-E09381h

Sensitivity

0.156 ng/ml

Detection Range

0.625 ng/ml-40 ng/ml

Reactivity

Human

Assay Duration

1-5h

Alternative Names

serpin peptidase inhibitor, clade B (ovalbumin), member 3, HsT1196, SCC, SCCA-1, SCCA-PD, SCCA1, T4-A, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3, squamous cell carcinoma antigen 1

Recovery

The recovery of human CLEC9A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	106	103-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC9A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	104
	Range %	100-108
1:4	Average %	89
	Range %	85-93
1:8	Average %	108
	Range %	104-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.273	2.109	2.191	2.038
10	1.575	1.604	1.590	1.437
5	1.179	1.205	1.192	1.039
2.5	0.825	0.825	0.825	0.672
1.25	0.525	0.532	0.529	0.376
0.625	0.375	0.368	0.372	0.219
0.312	0.264	0.261	0.263	0.110
0	0.154	0.152	0.153	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P29508

Applications	ELISA
Research Area	SERPINB3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	SCCA, TSGF, and the Long Non-Coding RNA AC007271.3 are Effective Biomarkers for Diagnosing Oral Squamous Cell Carcinoma Shao T.et al,Cell Physiol Biochem,May 9

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SKU	Supplier No.	Size	Your price
BHE10504437	CSB-E09381h	96T	$790	Add to Cart
BHE10504437	CSB-E09381h	5x96T	$3792	Add to Cart
BHE10504437	CSB-E09381h	10x96T	$7505	Add to Cart

Human squamous cell carcinoma antigen 1 (SCCAg1) ELISA kit

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