JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human Protein prune homolog 2 (PRUNE2) ELISA Kit

SKU : BHE13703313

The deduced 2,714-amino acid protein has a coiled-coil sequence, a proline-rich region, a P loop, and a C-terminal BNIP2 and CDC42GAP (ARHGAP1) homology (BCH) domain, which contains 3 putative transmembrane domains. Northern blot analysis detected a 12-kb transcript in fetal brain. Semiquantitative RT-PCR revealed BMCC1 expression in all tissues examined except bone marrow, thymus, and spleen.
Expression was highest in brain, cerebellum, spinal cord, and adrenal gland. RT-PCR of synchronized HeLa cells showed that BMCC1 was predominantly expressed in G1 phase of the cell cycle. In situ hybridization of mouse embryos showed that Bmcc1 was specifically expressed in neural tube and neural crest-related tissues.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE61097
Description	This Human Protein prune homolog 2 (PRUNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRUNE2.
Alternative Names	PRUNE2; RP11-214N16.3; A214N16.3; BMCC1; BNIPXL; C9orf65; DKFZp762K117; FLJ50060; FLJ54876; FLJ59118; KIAA0367; RP11-58J3.2; bA214N16.3; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Protein prune homolog 2 microplate Human Protein prune homolog 2 standard Human Protein prune homolog 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein prune homolog 2 (PRUNE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRUNE2. No significant cross-reactivity or interference between Human PRUNE2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The deduced 2,714-amino acid protein has a coiled-coil sequence, a proline-rich region, a P loop, and a C-terminal BNIP2 and CDC42GAP (ARHGAP1) homology (BCH) domain, which contains 3 putative transmembrane domains. Northern blot analysis detected a 12-kb transcript in fetal brain. Semiquantitative RT-PCR revealed BMCC1 expression in all tissues examined except bone marrow, thymus, and spleen. Expression was highest in brain, cerebellum, spinal cord, and adrenal gland. RT-PCR of synchronized HeLa cells showed that BMCC1 was predominantly expressed in G1 phase of the cell cycle. In situ hybridization of mouse embryos showed that Bmcc1 was specifically expressed in neural tube and neural crest-related tissues.
Notes	This Human Protein prune homolog 2 (PRUNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRUNE2 in samples. An antibody specific for PRUNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRUNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRUNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRUNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	158471
Antigen	PRUNE2
Tag	PRUNE2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Protein prune homolog 2 (PRUNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRUNE2 in samples. An antibody specific for PRUNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRUNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRUNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRUNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13703313	KTE61097	48 T	$339		Add to Cart
BHE13703313	KTE61097	96 T	$589		Add to Cart
BHE13703313	KTE61097	96 Tx5	$2629		Add to Cart
BHE13703313	KTE61097	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.