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Home / ELISA Kit

Human Prominin 2 (PROM2) ELISA Kit

SKU : BHE13703339

Prominin 2 is a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants.Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells
Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.

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Mfr.No.	KTE61123
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prominin 2 (PROM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROM2.
Alternative Names	PROM2; MGC138714; PROML2; OTTHUMP00000207357; prominin-like protein 2; prominin-related protein
Detection Method	Colorimetric
Kit Components	Human Prominin 2 microplate Human Prominin 2 standard Human Prominin 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prominin 2 (PROM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PROM2. No significant cross-reactivity or interference between Human PROM2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prominin 2 is a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants.Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.
Notes	This Human Prominin 2 (PROM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROM2 in samples. An antibody specific for PROM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	150696

Applications	ELISA
Applications Notes	This Human Prominin 2 (PROM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROM2 in samples. An antibody specific for PROM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703339	KTE61123	48 T	$359		Add to Cart
BHE13703339	KTE61123	96 T	$619		Add to Cart
BHE13703339	KTE61123	96 Tx5	$2809		Add to Cart
BHE13703339	KTE61123	96 Tx50	$26209		Add to Cart

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