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Home / ELISA Kit

Human Probable peptidyl-tRNA hydrolase (PTRH1) ELISA Kit

SKU : BHE13703257

PTRH1, Belongs to the PTH family.Peptidyl-tRNA hydrolase (Pth) activity releases tRNA from the premature translation termination product peptidyl-tRNA. Two different enzymes have been reported to encode such activity, Pth present in bacteria and eukaryotes and Pth2 present in archaea and eukaryotes.
Prokaryotic and eukaryotic cells show an enzymatic activity that releases the peptidyl moiety from the tRNA, called peptidyl-tRNA hydrolase (Pth), allowing the free tRNA and peptide to be reused in protein synthesis. First identified in Escherichia coli, genes encoding Pth have been found in bacteria and eukaryotes but not in archaea. E. coli Pth crystal structure has been solved and exhibits an alpha/beta fold formed by three layers with a mixed five-strand beta-sheet at the core.

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Mfr.No.	KTE61041
Description	This Human Probable peptidyl-tRNA hydrolase (PTRH1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTRH1.
Alternative Names	PTRH1; C9orf115; MGC51999; PTH1; peptidyl-tRNA hydrolase 1 homolog
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Probable peptidyl-tRNA hydrolase microplate Human Probable peptidyl-tRNA hydrolase standard Human Probable peptidyl-tRNA hydrolase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Probable peptidyl-tRNA hydrolase (PTRH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTRH1. No significant cross-reactivity or interference between Human PTRH1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PTRH1, Belongs to the PTH family.Peptidyl-tRNA hydrolase (Pth) activity releases tRNA from the premature translation termination product peptidyl-tRNA. Two different enzymes have been reported to encode such activity, Pth present in bacteria and eukaryotes and Pth2 present in archaea and eukaryotes. Prokaryotic and eukaryotic cells show an enzymatic activity that releases the peptidyl moiety from the tRNA, called peptidyl-tRNA hydrolase (Pth), allowing the free tRNA and peptide to be reused in protein synthesis. First identified in Escherichia coli, genes encoding Pth have been found in bacteria and eukaryotes but not in archaea. E. coli Pth crystal structure has been solved and exhibits an alpha/beta fold formed by three layers with a mixed five-strand beta-sheet at the core.
Notes	This Human Probable peptidyl-tRNA hydrolase (PTRH1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTRH1 in samples. An antibody specific for PTRH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTRH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTRH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTRH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	138428
Antigen	Ptrh1
Tag	PTRH1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Probable peptidyl-tRNA hydrolase (PTRH1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTRH1 in samples. An antibody specific for PTRH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTRH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTRH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTRH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703257	KTE61041	48 T	$339		Add to Cart
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BHE13703257	KTE61041	96 Tx50	$24569		Add to Cart

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