Matrices listed below were spiked with certain level of PAP and the recovery rates were calculated by comparing the measured value to the expected amount of PAP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
96
EDTA plasma(n=5)
86-99
93
heparin plasma(n=5)
88-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
85-102%
85-102%
EDTA plasma(n=5)
88-101%
82-99%
85-96%
heparin plasma(n=5)
86-92%
81-99%
83-95%
Sensitivity
< 37.5pg/ml
Notes
For Research Use Only
UniProt ID
P15309
Antigen
PAP
Applications
ELISA
Applications Notes
PAP ELISA Kit allows for the in vitro quantitative determination of PAP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PAP(Prostatic acid phosphatase) ELISA Kit