Human Metallothionein3 - MT-3 ELISA Kit

Mfr.No.

E4417Hu

Description

This sandwich kit is for the accurate quantitative detection of Metallothionein 3 (also known as MT-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GIF;GIFB;GRIF;Growth inhibitory factor;Metallothionein-3;Metallothionein-III;MT-3;MT-III;ZnMT3;

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

6927

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.023ng/ml

Background

Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.

Gene ID	4504
UniProt ID	P25713
Antigen	MT-3

Research Area

Neuroscience

Protocol

These standard curves of E4417Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.063	2.075	2.041
	2.088
3.2ng/ml	1.614	1.673	1.639
	1.733
1.6ng/ml	1.155	1.128	1.093
	1.1
0.8ng/ml	0.763	0.74	0.706
	0.718
0.4ng/ml	0.49	0.452	0.418
	0.415
0ng/ml	0.032	0.034	0
	0.036

Precision

We measured random samples of E4417Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.13	0.07	6.45
Intra-Assay	2	18	2.54	0.14	5.59
Intra-Assay	3	18	0.68	0.05	6.94

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105767	E4417Hu	48T	$320		Add to Cart
BHE12105767	E4417Hu	96T	$458		Add to Cart

Human Metallothionein3 - MT-3 ELISA Kit

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