Matrices listed below were spiked with certain level of MAD2L1 and the recovery rates were calculated by comparing the measured value to the expected amount of MAD2L1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-100
94
EDTA plasma(n=5)
86-102
97
heparin plasma(n=5)
85-105
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAD2L1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-97%
87-99%
89-105%
EDTA plasma(n=5)
82-98%
82-99%
82-101%
heparin plasma(n=5)
81-99%
82-89%
82-95%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
MAD2L1 ELISA Kit allows for the in vitro quantitative determination of MAD2L1 concentrations in serum, plasma, tissue homogenates and other biological fluids.