Human Interleukin-36 gamma(IL1F9) ELISA kit

Mfr.No.

CSB-EL011620HU

Alternative Names

interleukin 1 family, member 9, IL-1F9, IL-1H1, IL-1RP2, IL1E, IL1H1, IL1RP2, IL-1 related protein 2, IL-1(EPSILON), IL-1-epsilon, interleukin 1-related protein 2, interleukin-1 epsilon, interleukin-1 homolog 1

Reactivity

Human

Detection Range

78 pg/mL-5000 pg/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-107
EDTA plasma (n=4)	98	92-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	94
	Range %	90-98
1:2000	Average %	102
	Range %	96-107
1:4000	Average %	87
	Range %	81-92
1:8000	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
25	2.867	2.723	2.795	2.707
12.5	1.988	1.856	1.922	1.834
6.25	1.258	1.219	1.239	1.151
3.12	0.734	0.715	0.725	0.637
1.56	0.468	0.479	0.474	0.386
0.78	0.242	0.248	0.245	0.157
0.39	0.152	0.159	0.156	0.068
0	0.088	0.087	0.088	?

Species

Homo sapiens

Sensitivity

19.5 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9NZH8
Antigen	Interleukin 1 Family, Member 9

Applications

ELISA

Research Area

IL1F9

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503009	CSB-EL011620HU	96T	$695	Add to Cart
BHE10503009	CSB-EL011620HU	5x96T	$3336	Add to Cart
BHE10503009	CSB-EL011620HU	10x96T	$6602.50	Add to Cart

Human Interleukin-36 gamma(IL1F9) ELISA kit

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