Human Insulin-like Growth Factor 2 MRNA-binding Protein 2

Mfr.No.

E5473Hu

Alternative Names

Hepatocellular carcinoma autoantigen p62;IGF2 mRNA-binding protein 2;IGF2BP2;IGF-II mRNA-binding protein 2;IMP-2;Insulin-like growth factor 2 mRNA-binding protein 2;VICKZ family member 2

Reactivity

Human

Detection Range

70-15000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

66121

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

33.25ng/L

Background

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the Insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.

Gene ID	10644
UniProt ID	Q9Y6M1
Antigen	IGF2BP2

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E5473Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8000ng/L	2.307	2.264	2.234
	2.22
4000ng/L	1.232	1.216	1.187
	1.2
2000ng/L	0.697	0.631	0.602
	0.566
1000ng/L	0.402	0.392	0.363
	0.383
500ng/L	0.131	0.141	0.112
	0.152
0ng/L	0.016	0.029	0
	0.042

Precision

We measured random samples of E5473Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2132.2	117.28	5.5
Intra-Assay	2	18	4010	198.93	5
Intra-Assay	3	18	4194.8	108.34	2.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106835	E5473Hu	48T	$320		Add to Cart
BHE12106835	E5473Hu	96T	$458		Add to Cart

Human Insulin-like Growth Factor 2 MRNA-binding Protein 2 - IGF2BP2 ELISA Kit

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