Human Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2) ELISA kit

Mfr.No.

CSB-EL011091HU

Alternative Names

insulin-like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, VICKZ2, p62, IGF-II mRNA-binding protein 2

Reactivity

Human

Detection Range

125 pg/mL-8000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human MAGED2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	102-108
EDTA plasma (n=4)	96	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MAGED2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	93
	Range %	88-97
1:8	Average %	107
	Range %	102-111

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

31.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9Y6M1
Antigen	insulin-like growth factor 2 mRNA binding protein 2

Applications

ELISA

Research Area

IGF2BP2

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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