Matrices listed below were spiked with certain level of IA-2 Autoantibody and the recovery rates were calculated by comparing the measured value to the expected amount of IA-2 Autoantibody in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
98
EDTA plasma(n=5)
90-105
98
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IA-2 Autoantibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-95%
85-93%
85-104%
EDTA plasma(n=5)
86-99%
82-97%
84-101%
heparin plasma(n=5)
86-98%
85-96%
80-99%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IA-2 Autoantibody ELISA Kit allows for the in vitro quantitative determination of IA-2 Autoantibody concentrations in serum, plasma, tissue homogenates and other biological fluids.