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Home / ELISA Kit

Human heat shock protein 70(HSP-70) antibody (IgG) ELISA kit

SKU : BHE10502640

This Human GRP ELISA Kit was designed for the quantitative measurement of Human GRP protein in serum, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.312 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E14905h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

heat shock protein 70(HSP-70) antibody (IgG)

Recovery

The recovery of human GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-101
1:2		Average %		103
		Range %		98-110
1:4		Average %		92
		Range %		88-96
1:8		Average %		86
		Range %		80-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.694	2.572	2.633	2.512
40	2.082	2.105	2.094	1.973
20	1.418	1.476	1.447	1.326
10	0.891	0.835	0.863	0.742
5	0.540	0.572	0.556	0.435
2.5	0.350	0.341	0.346	0.225
1.25	0.276	0.262	0.269	0.148
0	0.124	0.117	0.121

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

Applications	ELISA
Research Area	HSP70 Ab

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10502640	CSB-E14905h	96T	$695		Add to Cart
BHE10502640	CSB-E14905h	5x96T	$3336		Add to Cart
BHE10502640	CSB-E14905h	10x96T	$6602.50		Add to Cart

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