Matrices listed below were spiked with certain level of HD3 and the recovery rates were calculated by comparing the measured value to the expected amount of HD3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
94
EDTA plasma(n=5)
85-95
93
heparin plasma(n=5)
90-99
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HD3 ELISA Kit allows for the in vitro quantitative determination of HD3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HD3 (Histone Deacetylase 3) ELISA Kit