Human Grip and Coiled-Coil Domain-containing Protein 2

Mfr.No.

E3650Hu

Description

This sandwich kit is for the accurate quantitative detection of human GRIP and Coiled-coil Domain-containing Protein 2 (also known as GCC2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

185 kDa Golgi coiled-coil protein;CLL-associated antigen KW-11;CTCL tumor antigen se1-1;GCC 2;GCC185;GCC2;GRIP and coiled-coil domain-containing protein 2;Ran-binding protein 2-like 4;RanBP2L4;Renal carcinoma antigen NY-REN-53

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

195910

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.024ng/ml

Background

Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2.

Gene ID	9648
UniProt ID	Q8IWJ2
Antigen	GCC2

Research Area

Others

Protocol

These standard curves of E3650Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
7.2ng/ml	1.871	1.907	1.871
	1.943
3.6ng/ml	0.936	0.977	0.942
	1.018
1.8ng/ml	0.58	0.589	0.553
	0.598
0.9ng/ml	0.216	0.238	0.203
	0.261
0.45ng/ml	0.142	0.11	0.074
	0.078
0ng/ml	0.047	0.036	0
	0.024

Precision

We measured random samples of E3650Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.8	0.15	3.2
Intra-Assay	2	18	2.2	0.11	5.2
Intra-Assay	3	18	4.3	0.23	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105124	E3650Hu	48T	$320		Add to Cart
BHE12105124	E3650Hu	96T	$458		Add to Cart

Human Grip and Coiled-Coil Domain-containing Protein 2 - GCC2 ELISA Kit

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