Human Gremlin 1 - GREM1 ELISA Kit

Mfr.No.

E2735Hu

Description

This sandwich kit is for the accurate quantitative detection of human Gremlin 1 (also known as GREM1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cell proliferation-inducing gene 2 protein;Cysteine knot superfamily 1, BMP antagonist 1;DAN domain family member 2;Down-regulated in Mos-transformed cells protein;GREM 1;GREM1;Gremlin-1;IHG-2;Increased in high glucose protein 2;

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

20697

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.025ng/ml

Background

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner (By similarity). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (PubMed: 27036124). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed (By similarity).

Gene ID	26585
UniProt ID	O60565
Antigen	GREM1

Research Area

Signal Transduction

Protocol

These standard curves of E2735Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.208	2.152	2.075
	2.096
6ng/ml	1.23	1.269	1.192
	1.307
3ng/ml	0.687	0.768	0.691
	0.848
1.5ng/ml	0.512	0.47	0.394
	0.428
0.75ng/ml	0.306	0.298	0.222
	0.291
0ng/ml	0.074	0.076	0
	0.079

Precision

We measured random samples of E2735Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.5	0.29	5.3
Intra-Assay	2	18	7.8	0.29	3.8
Intra-Assay	3	18	1.5	0.11	7.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104273	E2735Hu	48T	$320		Add to Cart
BHE12104273	E2735Hu	96T	$458		Add to Cart

Human Gremlin 1 - GREM1 ELISA Kit

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