Human FAM3A(Family With Sequence Similarity 3, Member A) ELISA Kit

Mfr.No.

ELK5320

Alternative Names

XAP-7; Cytokine-like protein 2-19

Reactivity

Human

Detection Range

15.63-1000 pg/mL

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant FAM3A and the recovery rates were calculated by comparing the measured value to the expected amount of FAM3A in samples.

Matrix	Recovery range	Average
serum(n=5)	96-107%	101%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	85-97%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FAM3A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	82-94%	85-96%	78-90%	91-99%
EDTA plasma(n=5)	87-96%	92-105%	89-102%	92-101%
Heparin plasma(n=5)	85-96%	87-101%	88-102%	87-102%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.154	2.067
500.00	1.636	1.549
250.00	1.037	0.950
125.00	0.671	0.584
62.50	0.508	0.421
31.25	0.362	0.275
15.63	0.283	0.196
0.00	0.087	0.000

Sensitivity

26.1 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	P98173
Antigen	FAM3A
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FAM3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FAM3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FAM3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FAM3A in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Signal transduction;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15201473	ELK5320	48T	$320		Add to Cart
BHE15201473	ELK5320	96T	$458		Add to Cart

Human FAM3A(Family With Sequence Similarity 3, Member A) ELISA Kit

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