Human Eukaryotic Translation Initiation Factor 4B

Mfr.No.

E5267Hu

Sensitivity

7.81ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

EIF 4B;EIF4B;eIF-4B;Eukaryotic translation initiation factor 4B

Molecular Weight

69151

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5267Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	699	28.02	4
Intra-Assay	2	18	1327.2	36.56	2.8
Intra-Assay	3	18	244.4	13.83	5.7

Background

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Protocol

These standard curves of E5267Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.047	2.155	2.111
	2.262
800ng/L	1.144	1.223	1.18
	1.302
400ng/L	0.633	0.703	0.659
	0.772
200ng/L	0.519	0.461	0.417
	0.403
100ng/L	0.188	0.207	0.164
	0.226
0ng/L	0.042	0.043	0
	0.045

Gene ID	1975
UniProt ID	P23588

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106630	E5267Hu	48T	$320		Add to Cart
BHE12106630	E5267Hu	96T	$458		Add to Cart

Human Eukaryotic Translation Initiation Factor 4B - EIF4B ELISA Kit

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