Human Epithelial cell adhesion molecule(EPCAM) ELISA kit

Human Epithelial cell adhesion molecule(EPCAM) ELISA kit

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Mfr.No.

CSB-EL007717HU

Alternative Names

epithelial cell adhesion molecule, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, KS1/4, KSA, M4S1, MIC18, MK-1, TACST-1, TACSTD1, TROP1, hEGP-2, adenocarcinoma-associated antigen, carcinoma-associated antigen GA733-

Reactivity

Human

Detection Range

0.625 ng/ml-40 ng/ml

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of rat NGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	98	94-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	99
	Range %	96-102
1:4	Average %	93
	Range %	90-96
1:8	Average %	88
	Range %	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.256	2.104	2.180	2.035
500	1.678	1.637	1.658	1.513
250	1.131	1.168	1.150	1.005
125	0.742	0.765	0.754	0.609
62.5	0.510	0.500	0.505	0.360
31.2	0.416	0.401	0.409	0.264
15.6	0.301	0.311	0.306	0.161
0	0.144	0.146	0.145

Species

Homo sapiens

Sensitivity

0.156 ng/ml

Limit Of Detection

450 nm

UniProt ID	Q3T0L5
Antigen	Epithelial Cell Adhesion Molecule

Applications

ELISA

Research Area

EPCAM

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502291	CSB-EL007717HU	96T	$695	Add to Cart
BHE10502291	CSB-EL007717HU	5x96T	$3336	Add to Cart
BHE10502291	CSB-EL007717HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.