Human Endothelin 1 - ET-1 ELISA Kit

Mfr.No.

E1238Hu

Sensitivity

1.01ng/L

Detection Range

2-600ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Endothelin 1 (also known as ET-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Big endothelin-1;EDN 1;EDN1;Endothelin-1;ET-1;PPET1;Preproendothelin-1

Molecular Weight

24425

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1238Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	194.2	6.49	3.3
Intra-Assay	2	18	96	4.32	4.5
Intra-Assay	3	18	64.5	3.78	5.9

Background

Endothelins are endothelium-derived vasoconstrictor Peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed: 19086031).

Protocol

These standard curves of E1238Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.504	2.438	2.384
	2.372
160ng/L	1.52	1.467	1.413
	1.413
80ng/L	0.736	0.778	0.725
	0.82
40ng/L	0.439	0.458	0.404
	0.477
20ng/L	0.192	0.207	0.153
	0.221
0ng/L	0.043	0.053	0
	0.063

Gene ID	1906
UniProt ID	P05305

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102884	E1238Hu	48T	$320		Add to Cart
BHE12102884	E1238Hu	96T	$458		Add to Cart

Human Endothelin 1 - ET-1 ELISA Kit

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