Human corticotropin releasing hormone,CRH ELISA Kit

Mfr.No.

CSB-E06872h

Alternative Names

corticotropin releasing hormone, CRF, corticotropin-releasing factor

Reactivity

Human

Detection Range

1 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of dog EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	83-102
EDTA plasma (n=4)	95	85-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-105
1:2	Average %	89
	Range %	80-97
1:4	Average %	93
	Range %	86-100
1:8	Average %	95
	Range %	88-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

1 ng/mL

Limit Of Detection

450 nm

UniProt ID	P06850
Antigen	Corticotropin Releasing Hormone

Applications

ELISA

Research Area

CRH

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501954	CSB-E06872h	96T	$790	Add to Cart
BHE10501954	CSB-E06872h	5x96T	$3792	Add to Cart
BHE10501954	CSB-E06872h	10x96T	$7505	Add to Cart