Matrices listed below were spiked with certain level of COL9A3 and the recovery rates were calculated by comparing the measured value to the expected amount of COL9A3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
93
EDTA plasma(n=5)
89-103
97
heparin plasma(n=5)
92-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL9A3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
COL9A3 chain) ELISA Kit allows for the in vitro quantitative determination of COL9A3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human COL9A3(Collagen alpha-3(IX) chain) ELISA Kit