Human Bh3 Interacting Domain Death Agonist

Mfr.No.

E1831Hu

Description

This sandwich kit is for the accurate quantitative detection of human BH3 Interacting Domain Death Agonist (also known as BID) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BH3-interacting domain death agonist;BH3-interacting domain death agonist p11;BH3-interacting domain death agonist p13;BH3-interacting domain death agonist p15;Bid;FP497;p11 BID);p13 BID;p15 BID;p22 BID

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

21995

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.025ng/ml

Background

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Gene ID	637
UniProt ID	P55957
Antigen	Bid

Research Area

Cell Biology

Protocol

These standard curves of E1831Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.554	2.473	2.428
	2.393
6ng/ml	1.475	1.501	1.456
	1.528
3ng/ml	0.904	0.925	0.879
	0.946
1.5ng/ml	0.518	0.523	0.477
	0.529
0.75ng/ml	0.232	0.213	0.167
	0.194
0ng/ml	0.028	0.046	0
	0.063

Precision

We measured random samples of E1831Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.85	0.21	3.56
Intra-Assay	2	18	1.31	0.09	6.8
Intra-Assay	3	18	2.57	0.14	5.34

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103405	E1831Hu	48T	$320		Add to Cart
BHE12103405	E1831Hu	96T	$458		Add to Cart

Human Bh3 Interacting Domain Death Agonist - BID ELISA Kit

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