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Home / ELISA Kit

EliKine Rat TGF-beta1 ELISA Kit

SKU : BHE13710199

Transforming growth factor beta (TGF beta) is a pleiotropic cytokine that exhibits a broad spectrum of biological and regulatory effects on the cellular and organism level. It plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels (e.g. mRNA turnover, latent protein activation or post-translational modifications).

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Mfr.No.	KTE9006
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	EliKine Rat TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate Rat TGF-beta1.
Alternative Names	TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide
Detection Method	Colorimetric
Kit Components	Rat TGF-beta1 microplate Rat TGF-beta1 standard Rat TGF-beta1 detect antibody EliKine Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	EliKine Rat TGF-beta1 ELISA Kit has high sensitivity and excellent specificity for detection of Rat TGF-beta1. No significant cross-reactivity or interference between Rat TGF-beta1 and analogues was observed.
Calibration Range	15.6 pg/mL-1000 pg/mL
Limit Of Detection	8 pg/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Transforming growth factor beta (TGF beta) is a pleiotropic cytokine that exhibits a broad spectrum of biological and regulatory effects on the cellular and organism level. It plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels (e.g. mRNA turnover, latent protein activation or post-translational modifications).
Notes	EliKine Rat TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-beta1 in samples. An antibody specific for TGF-beta1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-beta1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-beta1 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-beta1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	59086

Applications	ELISA
Applications Notes	EliKine Rat TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-beta1 in samples. An antibody specific for TGF-beta1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-beta1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-beta1 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-beta1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710199	KTE9006	48 T	$189		Add to Cart
BHE13710199	KTE9006	96 T	$289		Add to Cart

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