Dog Interleukin-1 receptor antagonist protein(IL1RN) ELISA kit

Dog Interleukin-1 receptor antagonist protein(IL1RN) ELISA kit

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Mfr.No.

CSB-EL011628DO

Alternative Names

interleukin 1 receptor antagonist, DIRA, ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430, MVCD4, IL1RN (IL1F3), OTTHUMP00000203730, intracellular IL-1 receptor antagonist type II, intracellular interleukin-1 receptor antagonist (icIL-1r

Reactivity

Canis familiaris, Dog

Detection Range

15.6 pg/ml-1000 pg/ml

Sample Type

Tissue homogenates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of rat TGF-?1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-104
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TGF-?1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-92
1:200	Average %	94
	Range %	89-99
1:400	Average %	95
	Range %	91-101
1:800	Average %	102
	Range %	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.560	2.589	2.575	2.388
200	2.262	2.299	2.281	2.094
100	1.787	1.798	1.793	1.606
50	1.098	1.076	1.087	0.900
25	0.581	0.598	0.590	0.403
12.5	0.421	0.452	0.437	0.250
6.25	0.307	0.321	0.314	0.127
0	0.186	0.188	0.187	?

Species

Canis lupus familiaris

Sensitivity

3.9 pg/ml

Limit Of Detection

450 nm

UniProt ID	Q9BEH0
Antigen	Interleukin 1 Receptor Antagonist

Applications

ELISA

Research Area

IL1RN

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500597	CSB-EL011628DO	96T	$595	Add to Cart
BHE10500597	CSB-EL011628DO	5x96T	$2856	Add to Cart
BHE10500597	CSB-EL011628DO	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.