WB: 1/500 - 1/5000, IHC: 1/50 - 1/200. Optimal dilutions/concentrations should b e determined by the end user.
Description
EIF4E (pS209) Antibody is a Monoclonal Antibody against EIF4E.
Immunohistochemistry analysis of EIF4E (pS209) Antibody diluted at 1/100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4?C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.