Chicken Tumor necrosis factor alpha,TNF-alpha ELISA KIT

Chicken Tumor necrosis factor alpha,TNF-alpha ELISA KIT

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Mfr.No.

CSB-E11231Ch

Alternative Names

Tumor necrosis factor alpha,TNF-alpha, DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha

Reactivity

Chicken

Detection Range

0.27 pg/mL-200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human REG1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	102	96-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human REG1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	85
	Range %	81-89
1:4	Average %	93
	Range %	83-97
1:8	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.608	2.514	2.561	2.426
5	1.893	1.787	1.840	1.705
2.5	1.147	1.085	1.116	0.981
1.25	0.627	0.607	0.617	0.482
0.625	0.374	0.367	0.371	0.236
0.312	0.287	0.293	0.290	0.155
0.156	0.231	0.226	0.229	0.094
0	0.136	0.134	0.135

Species

Gallus gallus

Sensitivity

0.27 pg/mL

Limit Of Detection

450 nm

Antigen	Tumor necrosis factor α,TNF-α

Applications

ELISA

Research Area

TNF-alpha

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by in broilers S Wang. et al,Journal of Animal Science,2015 Oct;93(10):4750-60 Effect of Antibiotic Growth Promoters on Anti-oxidative and Anti-inflammatory Activities in Broiler Chickens Kabploy K.et al,The Thai Journal of Veterinary Medicine,2016 46(1): 89 The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds Alhenaky A.et al,J Therm Biol.,Dec Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status, and immune response of laying hens in late Qu, X. Y.et al,Livestock Science,Jan The protective role of selenium against cadmium-induced hepatotoxicity in laying hens: Expression of Hsps and inflammation-related genes and modulation of elements homeostasis RunxiangZhang.et al,Ecotoxicology and Environmental Safety,May 21 Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,March 5 Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,January 7 Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti-Inflammatory Properties Sitong Zhang, et al,Oxidative Medicine and Cellular Longevity,December 30

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.