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Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line

SKU : BHC10901501

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T6080
Description	The Stable Bcl-xL Mouse Embryonic Fibroblast (Cytosol) Cell Line was derived from embryos of a pregnant mouse heterozygous for the bcl-x gene (bcl-x+/?). After immortalization by transfection of the SV40 large T antigen, the mouse embryonic fibroblasts were transfected with mutant Bcl-xL cDNA lacking the membrane-anchoring region. This stable cell line is able to express B-cell lymphoma-extra-large (Bcl-xL) which specifically confines to the cytosol. Bcl-xL is a major regulator of cellular apoptotic signalling by expressing Bcl-2 proteins. The Stable Bcl-xL Mouse Embryonic Fibroblast (Cytosol) Cell Line is a useful model in studying apoptosis regulation of tissue differentiation and homeostasis focused specifically in the cytosol. This cell line can also be useful in screening and development of new therapeutic strategies for cancer and other diseases.
Cell Type	Drug Discovery Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Growth Properties	Adherent
Quantity	1x106 cells / 1.0 ml
Tissue	Reproductive
Morphology	Polygonal
Species	Mouse (M. musculus)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Quality Control	1) Western blot analysis of Bcl-x expression; 2) Microarray analysis

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SKU	Supplier No.	Your price	Quantity
BHC10901501	T6080	$3950		Add to Cart

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