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Home / Cells / Cell Lines

MAPRE1 Doxycycline Inducible Knockout HeLa Cell Line

SKU : BHC10901309

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Applied Biological Materials (abm) Inc.

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Description
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Mfr.No.	T6173
Description	This human cell line stably expresses single guide RNA (sgRNA) targeting an early exon of MAPRE1 (microtubule-associated protein RP/EB family member 1) and of MAPRE3 (microtubule-associated protein RP/EB family member 3). Cas9 expression is induced by the presence of doxycycline enabling inducible knockout of the genes of interest. A PiggyBac transposon system was used to introduce Cas9 while guide RNAs were introduced using lentiviral infection.
Cell Type	Drug Discovery Cell Lines
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	EB1/MAPRE1 and EB3/MAPRE3; doxycycline-inducible Cas9 nuclease
Quantity	1x106 cells / 1.0 ml
Tissue	Cervix
Morphology	Epithelial-like
Population Doubling	24 - 34 hours
Species	Human (H. sapiens)
Quality Control	1) Western blot; 2) Immunofluorescence

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SKU	Supplier No.	Your price	Quantity
BHC10901309	T6173	$3950		Add to Cart

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