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Immortalized Rat Brain Microvascular Endothelial Cells - SV40T

SKU : BHC10900894

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Applied Biological Materials (abm) Inc.

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Description
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Mfr.No.	T9212
Description	The blood-brain barrier protects the central nervous system from potentially harmful substances by controlling the passage of cells and molecules. This barrier consists of vascular tissue, without pores, where the endothelial cells form tight junctions that allow only for select molecules to pass via pinocytosis. Immortalized Rat Brain Vascular Endothelial Cells (GPNT) was derived from microvessels in the cerebral cortex. The cells were immortalized by lipofection using an SV40 retroviral vector with a temperature sensitive large T antigen (tsA58) and contain a neomycin resistance gene. This cell line retains many characteristics of its primary parent cell line including expression of central nervous system specific markers, such as P-glycoprotein and GLUT-1, a high affinity glucose transporter not found in other vascular endothelial tissue. In addition, this cell line also expresses important immunological molecules including VCAM-I, PECAM-1 and MHC class I and II. Due to these properties this cell line may be useful in investigating immunological function, signal transduction, and migration of immune cells in specialized brain vascular endothelial tissue. More generally this cell line is ideal for research on the blood brain barrier.
Cell Type	Immortalized Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	P-glycoprotein, GLUT-1, the transferrin receptor, von Willebrand factor, RECA-1
Quantity	1x106 cells / 1.0 ml
Tissue	Blood Vessel
Morphology	Epithelial
Population Doubling	48 - 58 hours
Species	Rat (R. norvegicus)
Propagation	Use of Applied Cell Extracellular Matrix (G422) is required for celladhesion to the culture vessels. Grow cells in coated culture vessels with the followingconditions.The base medium for this cell line is Hams F10 Medium (Lonza). To make the completegrowth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999)to a final concentration of 10%, L-glutamine (G275) to a finalconcentration of 2 mM, Recombinant Rat FGF2 (Z300005)to a final concentration of 2 ng/mL, and heparin to a final concentration of 80 mug/mL.Changemedia every 2-3 days.Carbon dioxide (CO2): 5%,Temperature: 37.0°C.

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SKU	Supplier No.	Your price	Quantity
BHC10900894	T9212	$3950		Add to Cart

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