The Immortalized Mouse Preadipocyte Cells (ScAP-23) was isolated from subcutaneous adipose tissue from C57BL/6 mice and immortalized by infection with retroviruses carrying hTERT gene. The ScAP-23 preadipocytes convert to adipocytes when treated with dexamethasone, 3-methylisobutylxanthine, insulin, and indomethacin. Expression of key adipogenic transcription factors such as PPAR?, C/EBP family, SREBP-1c transcription factors and Krox20/Egr2 are found upregulated during ScAP-23 adipogenesis. The ScAP-23 adipocytes contain abundant lipid droplets and express transcripts for mature adipocytes markers such as SCD1, aFABP, ATGL, FAS, LDL, and GPDH. Furthermore, ScAP-23 adipocytes demonstrate insulin responsiveness and express insulin receptor-beta and major insulin-responsive glucose transporter GLUT4. Thus, the cell line is recommended as a tool for adipocytes biology and adipogenesis research.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Quantity
1x106 cells / 1.0 ml
Tissue
Adipose
Morphology
Fibroblast-like
Population Doubling
16 - 26 hours
Species
Mouse (M. musculus)
Propagation
Useof PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cellsin ECM-coated culture vessels unless otherwise specified in the Propagation Requirementsbelow.The base medium for this cell line is Prigrow III medium (TM003). To make the completed growthmedium, add the following components to the base medium at a final concentration of fetal bovine serum(TM999)*to a final concentration of 10%, 2mM L-glutamine (G275), and Penicillin/Streptomycin Solution(G255)to a final concentration of 1%.Carbon dioxide (CO2):5%, Temperature: 37.0°C.* abm does not recommend to use heat-inactivated FBS for cellculture unless specified otherwise.
Quality Control
1) Oil Red O staining at day 7 after inducing adipocyte differentiation to visualize lipid droplets; 2) Harvest RNA after induction of adipogenesis and perform RT-PCR and Northern Blot to detect transcript level of adipocyte markers SCD1, aFABP, ATGL, FAS, LDL, and GPDH B.
Reviews of Immortalized Mouse Pituitary Progenitor Cells (alphaT1-1)