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GLUT4-GFP Stable CHO Cell Line (CHO?HIRc?mycGLUT4eGFP)

SKU : BHC10901175

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T6498
Description	GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell line expresses GNAO1, the protein of which represent the alpha subunit of the G0 heterotrimeric G-protein signal-transducing complex and is partially responsible for smooth muscle contraction and depressing neuronal activity. This genes role is to inhibit adenylate cyclase , open K -channels (via beta/gamma subunits), and close Ca2 -channels. This particular cell line expresses the the Arginine243?? Histidine (R243H) mutation of GNAO1, which has been identified in breast carcinomas and is known to promote oncogenesis when introduced into cells. The oncogenic R234H mutation renders Galphao constitutively active by accelerating the rate of nucleotide exchange, which enhances signaling pathways that are associated with neoplasmic transformation. This cell line was made using retrovirus transduction. It carries flag tag and expresses GFP.
Cell Type	Drug Discovery Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	GNAO1 R243H, GFP
Quantity	1x106 cells / 1.0 ml
Tissue	Breast
Morphology	Epithelial-Like
Population Doubling	36 - 48 hours
Species	Human (H. sapiens)
Propagation	Use of PriCoat T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 50 µM fresh ascorbic acid + 2 nM estradiol + 1 µg/ml insulin + 2.8 µM hydrocortisone + 0.1 mM ethanolamine + 0.1 mM L-glutamine (G275), 15 nM sodium selenite + 1 ng/ml cholera toxin + 12.5 ng/ml hEGF (Z100139) + 10% fetal bovine serum (TM999) or 35 µg/ml bovine pituitary extract (Hammond) + 1% Penicillin/Streptomycin Solution (G255). Adjust media to pH 7.4 before use, 37.0°C, 5% CO?

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SKU	Supplier No.	Your price	Quantity
BHC10901175	T6498	$3950		Add to Cart

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