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FLNC CRISPR Knock Out A2780 Cell Line (Human)

SKU : BHC10901151

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T9659
Description	FLNC Stable Knockout Human A2780 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the FLNC gene (NM_001458) using the following guide RNA sequence: GCTGCTTGGGCGTCTGTTTG. FLNC is expressed in cardiac and skeletal muscles and thus can be used to study associated human cardiac diseases such as hypertrophic cardiomyophathy and restrictive cardiomyopathy. This cell line is sequenced verified to cause frameshift mutation.
Cell Type	Drug Discovery Cells
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Growth Properties	Adherent
Quantity	1x106 cells / 1.0 ml
Tissue	Reproductive
Morphology	Polygonal
Species	Human (H. sapiens)
Propagation	Use of PriCoatTMT25 Flasks (G299) or AppliedCell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Growcells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The basemedium for this cell line is Prigrow II medium available at ABM, Cat. No. TM002. To make the complete growth medium, add thefollowing components to the base medium: fetal bovine serum (TM999) to a finalconcentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.Selection: 1.0 mug/ml Puromycin (G264.)
Quality Control	1) 1.0 µg/ml of puromycin (G264) was used for clonal selection; 2) FLNC gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

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