Omega-3 polyunsaturated fatty acids (?3-PUFAs) are known to inhibit carcinogenesis, tumour growth and metastasis, whereas omega-6 polyunsaturated fatty acids (?6-PUFAs) stimulate their effects. The FAT1 gene of C. elegans encodes a ?3-desaturase that catalyses the conversion of ?6-PUFAs to ?3-PUFAs, increasing the amount of ?3-PUFAs. Fat-1 Stable RM1 Cell Line expresses the full-length FAT1 gene from C. elegans through stable transfection. These cells are applicable for studies investigating the effects of endogenous ?3-polyunsaturated fatty acids.
Biosafety Level
II
Density
25,000 - 35,000 cells/cm2
Growth Properties
Adherent
Quantity
1x106 cells / 1.0 ml
Morphology
Fibroblast-like
Organism
Mouse
Propagation
The base medium for this cell line is Prigrow I medium available at abm, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 20%, 12.5ug/ml endothelial-cell growth supplement (Sigma E2756), 0.05ug/ml heparin, 1X L-glutamine (G275), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Markers
FAT1
Source Organ
Prostate
Quality Control
1) RT-PCR to detect FAT1 gene
Shipping
Dry Ice
Storage
-180°C
Reference
Jing, K., Shin, S., & Lim, K. (2018). PO-123 Constitutive expression of FAT1 gene inhibits the invasion and tumorigenicity of cervical cancer cells through degradation of HPV E6/E7 oncoprotein via P53/RB in vitro and in vivo. ESMO Open, 3(Supplement 2), A69. https://doi.org/10.1136/esmoopen-2018-eacr25.164