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Cas9 Expressing THP-1 Cell Line

SKU : BHC10901715

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T3274
Description	THP-1 cells stably expressing Cas9
Biosafety Level	II
Density	100,000 - 200,000 cells/ml These cells can take up to a week to recover from thaw. We suggest using 20% FBS during this initial recovery
Freeze Thaw Recovery	1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media. 5. Centrifuge cells at 200x g for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO₂.
Growth Properties	Suspension
Quantity	1x10⁶ cells / 1.0 ml
Morphology	Round
Notes	Special ATCC MTA
Organism	Human
Population Doubling	24 - 36 hours
Propagation	The base medium for this cell line is Prigrow II medium available at abm Cat. No. TM002. To make the completed growth medium, add the following components to the base medium with the final concentration: fetal bovine serum (TM999) to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 1.2 µg/ml Puromycin (G264). Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Markers	Puromycin
Source Organ	Peripheral Blood
Quality Control	1) Puromycin selection 2) Western Blot analysis for Cas9 transgene expression

Shipping	Dry Ice
Storage	-180°C

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SKU	Supplier No.	Your price	Quantity
BHC10901715	T3274	$795		Add to Cart

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