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Cas9 Expressing 293T Cell Line

SKU : BHC10901695

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T3251
Description	HEK293T cells stably expressing Cas9
Biosafety Level	II
Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Freeze Thaw Recovery	1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media. 5. Centrifuge cells at 200x g for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO₂.
Growth Properties	Adherent
Quantity	1x10⁶ cells / 1.0 ml
Morphology	Polygonal
Organism	Human
Propagation	The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, 0.8 µg/ml puromycin (G264), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Markers	Puromycin resistance, neomycin resistance
Source Organ	Kidney
Quality Control	1) Puromycin selection 2) Western Blot analysis for Cas9 transgene expression

Shipping	Dry Ice
Storage	-180°C

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SKU	Supplier No.	Your price	Quantity
BHC10901695	T3251	$795		Add to Cart

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