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CRISPRa (SAM) MCF7 Cell Line

SKU : BHC18200146

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BPS Bioscience

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Mfr.No.	78522
Description	This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express a construct consisting of P65 (Transcription Factor p65, or Nuclear Factor NF-kappa-B p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag (from the MS2 coat protein), which is maintained with Hygromycin resistance. When these cells are transfected with an MS2 aptamer fused to a single guide RNA (sgRNA) targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the promoter in the genomic DNA by the MS2 aptamer and begin transcription, inducing high level expression of the desired protein.Cells were functionally validated by transfecting the cells with an sgRNA-MS2 targeting PD-1, resulting in high expression of PD-1. Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species. Host Species: human Supplied as Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (Biohippo #79796)
Application	Quickly generate CRISPR-activated cell pools or cell lines in MCF7 cells.Implement sgRNA CRISPRa screens in MCF7 cells.
Host Cell	MCF7 cells, human breast mammary gland cell line, epithelial cells, adherent
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected] Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200146	78522	2 vials	$2750		Add to Cart

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