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Home / Primary Antibodies

Bcl-2 Antibody (V2009)

SKU : BHA17111116

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NSJ Bioreagents

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Mfr.No.	V2009, V2009-20UG, V2009-100UG
Antigen	Bcl-2
Clonality	Monoclonal
Description	This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.
Isotype	IgG1,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Cytoplasmic, membrane

Gene Id	596
Clone No	124

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	FACS, IF, WB, IHC-P
Dilution	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Product Manual	Data Sheet

No Description Available.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17111116-1	V2009-20UG	20ug	$219		Add to Cart
BHA17111116-2	V2009-100UG	100ug	$499		Add to Cart

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