Rat TNF-alpha(Tumor Necrosis Factor Alpha) ELISA Kit

Mfr.No.

ER1393

Alternative Names

TNF-alpha ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

3.906-250pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of TNF-alpha and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-alpha in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-103	93
EDTA plasma(n=5)	86-104	95
heparin plasma(n=5)	88-104	94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-104%	88-103%	87-104%
EDTA plasma(n=5)	87-101%	84-100%	82-99%
heparin plasma(n=5)	87-100%	90-93%	82-99%

Sensitivity

< 2.344pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Notes

For Research Use Only

UniProt ID	P16599
Antigen	TNF-α

Applications	ELISA
Applications Notes	TNF-alpha ELISA Kit allows for the in vitro quantitative determination of TNF-alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: Asian Pacific Journal of Tropical Medicine Title: Ethanolic extracts of babandotan leaves (Ageratum conyzoides L.) prevents inflammation and proteoglycan degradation by inhibiting TNF-α and MMP-9 on osteoarthritis rats induced by monosodium iodoacetate Journal: European Journal of Inflammation Title: Hesperidin enhances angiogenesis via modulating expression of growth and inflammatory factor in diabetic foot ulcer in rats Journal: Cellular Physiology and Biochemistry Title: Blocking TRPA1 and TNF-α Signal Improves Bortezomib-Induced Neuropathic Pain Journal: Journal of Food and Nutrition Research Title: High Fiber Diet Suppression of TLR4 and NFκβ Gene Expression Correlated with TNF-α and IL-6 Levels in Hypertriglyceridemia Rats Journal: Molecular Pain Title: Neuroinflammation and central PI3K/Akt/mTOR signal pathway contribute to bone cancer pain

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE10805784	ER1393	96T	$350		Add to Cart

Rat TNF-alpha(Tumor Necrosis Factor Alpha) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat TNF-alpha(Tumor Necrosis Factor Alpha) ELISA Kit

Rate it now..!!