Rat SP-A1(Pulmonary surfactant-associated protein A1) CLIA Kit

Mfr.No.

E-CL-R0049

Antigen

SP-A

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
800	51069	54215	52642	52616
400	21149	22319	21734	21708
200	10231	9185	9708	9682
100	4273	4831	4552	4526
50	2320	2056	2188	2162
25	1094	1026	1060	1034
12.50	490	528	509	483
0	25	27	26	--

Specificity

This kit recognizes Rat SP-A in samples. No significant cross-reactivity or interference between Rat SP-A and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

12.5--800 pg/mL

Linearity

Samples were spiked with high concentrations of Rat SP-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	90-106	101-114	93-111
1:2	Average (%)	98	108	101
1:4	Range (%)	95-107	88-102	86-99
1:4	Average (%)	101	94	93
1:8	Range (%)	84-100	86-98	97-110
1:8	Average (%)	91	93	104
1:16	Range (%)	101-114	88-103	97-109
1:16	Average (%)	108	95	103

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat SP-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat SP-A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	40.83	119.10	317.22	38.48	112.90	345.99
Standard deviation	4.65	11.77	28.39	4.24	11.93	37.96
C V (%)	11.39	9.88	8.95	11.02	10.57	10.97

Recovery

The recovery of Rat SP-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	96-110	104
EDTA plasma (n=5)	96-111	103
Cell culture media (n=5)	89-101	95

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

7.5 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301925	E-CL-R0049	96T	$682	Add to Cart
BHE10301925	E-CL-R0049	96T*5	$3069	Add to Cart
BHE10301925	E-CL-R0049	96T*10	$5797	Add to Cart
BHE10301925	E-CL-R0049	48T	$545.60	Add to Cart

Rat SP-A1(Pulmonary surfactant-associated protein A1) CLIA Kit

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