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Home / Assay Kits / ELISA Kits

Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit

SKU : BHE13701104

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE100871
Antigen	POLR2E
Alternative Names	POLR2E ; RPABC1 ; RPB5 ; XAP4 ; hRPB25 ; hsRPB5 ; DNA directed RNA polymerase 23 kda polypeptide ; DNA directed RNA polymerase polypeptide E
Background	POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. A second site of hybridization to 11q23.1 was thought to represent a pseudogene, most likely a retroposon, since no remnants of introns could be identified in the study of genomic DNA.
Categories	Elisa Kit
Description	This Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2E.
Featured	Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit has high sensitivity and excellent specificity for detection of Rat POLR2E. No significant cross-reactivity or interference between Rat POLR2E and analogues was observed.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity	Rat
Tag	POLR2E
Description	This Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2E in samples. An antibody specific for POLR2E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR2E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2E bound in the initial step. The color development is stopped and the intensity of the color is measured.
Accession Number	B0BNE2
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich ELISA (quantitative)
Calibration Range	Please inquire
Detection Method	Colorimetric
Kit Components	• Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 microplate • Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 standard • Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit Of Detection	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma

Gene Id	690966
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.

Shipping	Gel pack with blue ice.
Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications	ELISA

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SKU	Supplier No.	Size	Your price	Quantity
BHE13701104	KTE100871	48T	$359.34		Add to Cart
BHE13701104	KTE100871	96T	$549.34		Add to Cart
BHE13701104	KTE100871	96T*5	$1935.73		Add to Cart
BHE13701104	KTE100871	96T*50	$17123.23		Add to Cart

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