Matrices listed below were spiked with certain level of MDC/CCL22 and the recovery rates were calculated by comparing the measured value to the expected amount of MDC/CCL22 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
95
EDTA plasma(n=5)
89-102
95
heparin plasma(n=5)
87-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MDC/CCL22 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MDC/CCL22 ELISA Kit allows for the in vitro quantitative determination of MDC/CCL22 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse MDC/CCL22(Macrophage-Derived Chemokine) ELISA Kit