Mouse APOE(Apolipoprotein E) CLIA Kit

Mfr.No.

E-CL-M0101

Antigen

APO-E

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
20	30741	37223	33982	33950
10	12209	12731	12470	12438
5	5397	5103	5250	5218
2.5	2495	2553	2524	2492
1.25	1486	1280	1383	1351
0.625	918	816	867	835
0.31	606	640	623	591
0	31	33	32	--

Specificity

This kit recognizes Mouse APO-E in samples. No significant cross-reactivity or interference between Mouse APO-E and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.313--20 ng/mL

Linearity

Samples were spiked with high concentrations of Mouse APO-E and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	97-108	91-103	87-101
1:2	Average (%)	103	97	94
1:4	Range (%)	88-101	97-108	92-105
1:4	Average (%)	94	102	99
1:8	Range (%)	97-112	88-102	101-119
1:8	Average (%)	104	95	109
1:16	Range (%)	87-100	84-95	89-100
1:16	Average (%)	93	90	94

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse APO-E were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse APO-E were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.02	3.21	8.72	1.00	3.53	9.37
Standard deviation	0.10	0.37	0.99	0.10	0.41	0.86
C V (%)	9.80	11.53	11.35	10.00	11.61	9.18

Recovery

The recovery of Mouse APO-E spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	98-113	106
EDTA plasma (n=5)	87-98	92
Cell culture media (n=5)	98-111	105

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.19 ng/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301359	E-CL-M0101	96T	$682	Add to Cart
BHE10301359	E-CL-M0101	96T*5	$3069	Add to Cart
BHE10301359	E-CL-M0101	96T*10	$5797	Add to Cart
BHE10301359	E-CL-M0101	48T	$545.60	Add to Cart

Mouse APOE(Apolipoprotein E) CLIA Kit

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