Human Rac1(Ras Related C3 Botulinum ToXin Substrate 1) CLIA Kit

Mfr.No.

E-CL-H1001

Antigen

RAC1

Alternative Names

Rac-1 ; TC-25 ; p21-Rac1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	53022	58420	55721	55693
1000	22555	25393	23974	23946
500	11598	10466	11032	11004
250	4857	5731	5294	5266
125	2807	2409	2608	2580
62.5	1343	1279	1311	1283
31.25	634	714	674	646
0	27	29	28	--

Specificity

This kit recognizes Human Rac1 in samples. No significant cross-reactivity or interference between Human Rac1 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Human Rac1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	94-112	95-109	86-100
1:2	Average (%)	102	102	92
1:4	Range (%)	85-97	96-108	100-117
1:4	Average (%)	92	102	108
1:8	Range (%)	87-104	99-117	102-115
1:8	Average (%)	95	108	109
1:16	Range (%)	85-99	101-115	94-107
1:16	Average (%)	92	108	100

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Rac1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Rac1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	94.61	179.58	767.05	102.69	184.23	807.22
Standard deviation	7.87	14.51	70.57	8.86	16.19	58.77
C V (%)	8.32	8.08	9.20	8.63	8.79	7.28

Recovery

The recovery of Human Rac1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	85-96	91
EDTA plasma (n=5)	85-98	91
Cell culture media (n=5)	101-116	107

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P63000

Manual

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SKU	Supplier No.	Size	Your price
BHE10300879	E-CL-H1001	96T	$682	Add to Cart
BHE10300879	E-CL-H1001	96T*5	$3069	Add to Cart
BHE10300879	E-CL-H1001	96T*10	$5797	Add to Cart
BHE10300879	E-CL-H1001	48T	$545.60	Add to Cart

Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) CLIA Kit

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