Human ENA-78/CXCL5(Epithelial Neutrophil ActIVating Peptide 78) ELISA Kit

Mfr.No.

E-EL-H0046

Antigen

ENA-78

Alternative Names

CXCL5 ; SCYB5

Background

This kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AMPK .Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMPK and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMPK ,biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The OD value is measured spectrophotometrically at a wavelength of 450nm. The OD value is positively associated with the concentration of Human AMPK . The concentration of Human AMPK in the samples can be calculated by comparing the OD of the samples to the standard curve.

Description

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	O.D	Average	Corrected
2000	2.475 2.489	2.482	2.395
1000	1.606 1.636	1.621	1.534
500	0.916 0.912	0.9139999999999999	0.827
250	0.501 0.531	0.516	0.429
125	0.298 0.292	0.295	0.208
62.5	0.195 0.181	0.188	0.101
31.25	0.134 0.144	0.139	0.052
0	0.078 0.096	0.08699999999999999	--

Specificity

This kit recognizes Human ENA-78 in samples. No significant cross-reactivity or interference between Human ENA-78 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Colormetric

Detection Range

31.25--2000pg/mL

Linearity

Samples were spiked with high concentrations of Human ENA-78 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	94-107	99-111	96-113
1:2	Average (%)	99	105	103
1:4	Range (%)	92-103	87-100	86-99
1:4	Average (%)	98	92	92
1:8	Range (%)	92-106	83-96	88-99
1:8	Average (%)	98	90	94
1:16	Range (%)	91-103	85-95	87-100
1:16	Average (%)	97	90	92

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ENA-78 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ENA-78 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	109.30	197.13	810.84	102.41	202.65	888.63
Standard	deviation	7.37	11.49	38.51	5.78	11.73	44.52
C V (%)	6.74	5.83	4.75	5.64	5.79	5.01

Recovery

The recovery of Human ENA-78 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	92-108	100
EDTA plasma (n=5)	88-102	93
Cell culture media (n=5)	95-110	101

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P42830

Manual

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SKU	Supplier No.	Size	Your price
BHE10302784	E-EL-H0046	96T	$609	Add to Cart
BHE10302784	E-EL-H0046	96T*5	$2740.50	Add to Cart
BHE10302784	E-EL-H0046	96T*10	$5176.50	Add to Cart
BHE10302784	E-EL-H0046	48T	$487.20	Add to Cart

Human ENA-78/CXCL5(Epithelial Neutrophil Activating Peptide 78) ELISA Kit

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