Matrices listed below were spiked with certain level of CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of CD25 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-99
94
EDTA plasma(n=5)
86-104
95
heparin plasma(n=5)
85-98
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CD25 ELISA Kit allows for the in vitro quantitative determination of CD25 concentrations in serum, plasma, tissue homogenates and other biological fluids.