Sensitive and accurate. Uses 10 uL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature. High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day.
Intended Use
OD340nm
Detection Principle
0.12 mM
Shelf Life
6 months
Specifity
For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism.
Reference
Moreno, D et al. (2020). Vulnerability of the endangered Maugean Skate population to degraded environmental conditions in Macquarie Harbour. Assay: Ketone body in maugean skate plasma.
Lin, X et al. (2020). Effects of dietary anaplerotic and ketogenic energy sources on renal fatty acid oxidation induced by clofibrate in suckling neonatal pigs. International Journal of Molecular Sciences, 21(3), 726. Assay: Ketone body in porcine kidney.
Wallenius, V et al. (2020). Suppression of enteroendocrine cell glucagon-like peptide (Glp)-1 release by fat-induced small intestinal ketogenesis: A mechanism targeted by Roux-en-Y gastric bypass surgery but not by preoperative very-low-calorie diet. Gut, 69(8), 1423-1431. Assay: Ketone body in human serum.
Khalil, HMA et al. (2020). Edible dairy formula fortified with coconut oil for neuroprotection against aluminium chloride-induced Alzheimer?s disease in rats. Journal of Functional Foods, 104296. Assay: Ketone body in rat serum.
Umbarawan, Y et al. (2020). Reduced fatty acid uptake aggravates cardiac contractile dysfunction in streptozotocin-induced diabetic cardiomyopathy. Scientific Reports, 10(1), 20809. Assay: Ketone body in mouse serum.
Moorhead, SG et al. (2020). Variation of body condition and plasma energy substrates with life stage, sex, and season in wild-sampled nurse sharks Ginglymostoma cirratum. Journal of Fish Biology. Assay: Ketone body in shark plasma.
Cui, A., Fan, H., Zhang, Y., Zhang, Y., Niu, D., Liu, S. & Liu, Y. (2019). Dexamethasone-induced Kruppel-like factor 9 expression promotes hepatic gluconeogenesis and hyperglycemia. The Journal of clinical investigation 130:2266-2278. Assay: Ketone body in mice serum.
Saito, T., Kuma, A., Sugiura, Y., Ichimura, Y., Obata, M., Kitamura, H. & Saito, I. (2019). Autophagy regulates lipid metabolism through selective turnover of NCoR1. Nature communications 10(1):1567. Assay: Ketone body in mice plasma.
Herrick, K. J., Hippen, A. R., Kalscheur, K. F., Schingoethe, D. J., Ranathunga, S. D., Anderson, J. L. & Van Eys, J. E. (2018). Infusion of butyrate affects plasma glucose, butyrate, and beta-hydroxybutyrate but not plasma insulin in lactating dairy cows. Journal of dairy science, 101(4), 3524-3536. Assay: Ketone body in cow plasma.
Iso, T., Haruyama, H., Sunaga, H., Matsui, H., Matsui, M., Tanaka, R. & Hanaoka, H. (2018). CD36 is indispensable for nutrient homeostasis and endurance exercise capacity during prolonged fasting. Physiological reports, 6(19), e13884. Assay: Ketone body in mice serum.
Lyons, K. (2018). Physiological consequences of environmental contamination in an elasmobranch with matrotrophic histotrophy, the Round Stingray (Urobatis halleri). Assay: Ketone body in stingrays plasma.
Bisgaier, Charles L., and Daniela Carmen Oniciu (2017). Treatment of NASH with gemcabene. U.S. Patent No. 9,849,104 Assay: Ketone body in mice plasma.
Goossens, C., Marques, M. B., Derde, S., Vander Perre, S., Dufour, T., Thiessen, S. E. & De Bock, K. (2017). Premorbid obesity, but not nutrition, prevents critical illness-induced muscle wasting and weakness. Journal of cachexia, sarcopenia and muscle, 8(1), 89-101. Assay: Ketone body in mice serum.
Herrick, K. J., Hippen, A. R., Kalscheur, K. F., Schingoethe, D. J., Casper, D. P., Moreland, S. C., & Van Eys, J. E. (2017). Single-dose infusion of sodium butyrate, but not lactose, increases plasma beta-hydroxybutyrate and insulin in lactating dairy cows. Journal of dairy science, 100(1), 757-768. Assay: Ketone body in cow plasma.
Driver, C. (2016). Metformin does not prevent glucose intolerance but improves renal function and reduces oxidative stress in type 1 diabetes (Doctoral dissertation). Assay: Ketone body in rat serum and urine.
Murunga, A. N., Miruka, D. O., Driver, C., Nkomo, F. S., Cobongela, S. Z., & Owira, P. M. (2016). Grapefruit derived flavonoid naringin improves ketoacidosis and lipid peroxidation in type 1 diabetes rat model. PloS one, 11(4), e0153241. Assay: Ketone body in rat serum and urine.
Neondo, M. A. (2016). The effects of naringin on glucose tolerance and ketoacidosis in type 1 diabetes (Doctoral dissertation). Assay: Ketone body in rat serum and urine.
Syamsunarno, MRAA et al (2014). Fatty Acid Binding Protein 4 and 5 Play a Crucial Role in Thermogenesis under the Conditions of Fasting and Cold Stress. PloS one 9.3: e90825. Assay: Ketone body in mouse serum.
Fujieda, Y et al (2013). Inflammation and Resolution Are Associated with Upregulation of Fatty Acid beta-Oxidation in Zymosan-Induced Peritonitis. PloS one 8.6: e66270. Assay: Ketone body in mouse liver tissue.
Martin-Murphy, BV et al (2013). Increased susceptibility of natural killer T-cell-deficient mice to acetaminophen-induced liver injury. Hepatology 57.4: 1575-1584. Assay: Ketone body in mouse serum.
Syamsunarno, MRAA et al (2013). A Critical Role of Fatty Acid Binding Protein 4 and 5 (FABP4/5) in the Systemic Response to Fasting. PloS one 8.11: e79386. Assay: Ketone body in mouse serum.
Qi S et al (2012). Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics. Hepatol Res 42(7):677-85. Assay: Ketone body in human serum.
Klusonova P et al (2011) Local metabolism of glucocorticoids in Prague hereditary hypertriglyceridemic rats--effect of hypertriglyceridemia and gender. Steroids 76(12):1252-9. Assay: Ketone Body in rat Hepatocyte culture.
Sharma NS et al (2011). Metabolic profiling based quantitative evaluation of hepatocellular metabolism in presence of adipocyte derived extracellular matrix. PLoS One 6(5):e20137. Assay: Ketone body in rat hepatocyte culture.
Sharma NS et al (2011). Metabolic profiling based quantitative evaluation of hepatocellular metabolism in presence of adipocyte derived extracellular matrix. PLoS One 6(5):e20137. Assay: Ketone Body in human serum.
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